5JQK

The Xray Crystal Structure of P. falciparum Aminopeptidase P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	146.740, 100.070, 106.660
Unit cell angles	90.00, 105.37, 90.00

Refinement procedure

Resolution	44.993 - 2.350
R-factor	0.1904
Rwork	0.189
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ctz
RMSD bond length	0.003
RMSD bond angle	0.851
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	70.750
High resolution limit [Å]	2.350
Number of reflections	229848
<I/σ(I)>	8.2
Completeness [%]	99.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	45% 2 methyl 2,4 pentanediol 0.1 M sodium cacodylate pH 6