5JQG
An apo tubulin-RB-TTL complex structure used for side-by-side comparison
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.050, 158.120, 180.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.220 - 2.240 |
| R-factor | 0.18238 |
| Rwork | 0.181 |
| R-free | 0.21247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i55 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.690 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.220 | 2.300 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Number of reflections | 144729 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 0.1M MES, 6% PEG4000, 5% Glycerol, 30mM CaCl2, 30mM MgCl2 |
