5JQ1
Efficient targeting of the asialoglycoprotein receptor by polyvalent display of a compact galactosamine mimic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.080, 59.490, 68.650 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 34.370 - 1.830 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.370 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.091 | 0.490 |
| Number of reflections | 29063 | |
| <I/σ(I)> | 15.8 | 2.1 |
| Completeness [%] | 88.8 | 99.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M cacodylate, pH 6.8-7.4, 0.1-0.2M (NH4)2SO4, 14-20% PEG 8000 |
