Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.010, 215.010, 142.860 |
| Unit cell angles | 90.00, 110.11, 90.00 |
Refinement procedure
| Resolution | 48.973 - 3.750 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.26820 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Refinement software | PHENIX ((dev_2376)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.980 |
| High resolution limit [Å] | 3.750 |
| Number of reflections | 56637 |
| <I/σ(I)> | 9.45 |
| Completeness [%] | 98.5 |
| Redundancy | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-HCl pH 8.0, 2% v/v 1,4-dioxane and 12-14% w/v PEG3350 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-HCl pH 8.0, 2% v/v 1,4-dioxane and 12-14% w/v PEG3350 |
