5JOW

Bacteroides ovatus Xyloglucan PUL GH43A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.920
Spacegroup name	P 21 2 21
Unit cell lengths	75.432, 91.172, 156.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.690 - 1.600
R-factor	0.12735
Rwork	0.125
R-free	0.16698
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GH43B to be deposited
RMSD bond length	0.009
RMSD bond angle	1.365
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.690	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.092	1.050
Number of reflections	142867
<I/σ(I)>	14.5	1.9
Completeness [%]	100.0	100
Redundancy	8.1	8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	292	0.1 M Tris pH 7.2 - 7.8, 0.18 M magnesium chloride and 12% (w/v) PEG-6000