5JNS

Crystal structure of human low molecular weight protein tyrosine phosphatase (LMPTP) type A complexed with phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0781
Spacegroup name	P 21 21 21
Unit cell lengths	32.210, 55.090, 95.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.680 - 1.800
R-factor	0.1669
Rwork	0.165
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jnr
RMSD bond length	0.030
RMSD bond angle	2.613
Data reduction software	XDS
Data scaling software	Aimless (0.5.9)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.090	55.090	1.770
High resolution limit [Å]	1.740	9.020	1.740
Rmerge	0.108	0.062	0.607
Number of reflections	18220
<I/σ(I)>	9.1
Completeness [%]	99.8	96.9	99.9
Redundancy	6.4	5.1	5.4
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	293	25% PEG 3350K, 200 mM NaCl