5JNR
Crystal structure of human low molecular weight protein tyrosine phosphatase (LMPTP) type A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.404, 55.863, 96.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.510 - 2.000 |
| R-factor | 0.1658 |
| Rwork | 0.163 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n8i |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.651 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.510 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.238 |
| Number of reflections | 12105 | |
| <I/σ(I)> | 34 | |
| Completeness [%] | 96.9 | 91.1 |
| Redundancy | 3.8 | 3.4 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 7% PEG 10K, Bis Tris, Ammonium Acetate |
