5JNR

Crystal structure of human low molecular weight protein tyrosine phosphatase (LMPTP) type A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	32.404, 55.863, 96.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.510 - 2.000
R-factor	0.1658
Rwork	0.163
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n8i
RMSD bond length	0.017
RMSD bond angle	1.651
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.510	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.064	0.238
Number of reflections	12105
<I/σ(I)>	34
Completeness [%]	96.9	91.1
Redundancy	3.8	3.4
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	293	7% PEG 10K, Bis Tris, Ammonium Acetate