5JNB
structure of GLD-2/RNP-8 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.347, 86.435, 91.907 |
| Unit cell angles | 89.88, 93.13, 90.22 |
Refinement procedure
| Resolution | 46.254 - 2.486 |
| R-factor | 0.1944 |
| Rwork | 0.192 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zrl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.260 |
| High resolution limit [Å] | 2.486 |
| Number of reflections | 135156 |
| <I/σ(I)> | 8.8 |
| Completeness [%] | 95.6 |
| Redundancy | 2.48 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 18 % (v/v) PEG MME 550, 50 mM Potassium Nitrate, 60 mM Magnesium Nitrate, 30 mM Hepes pH 7.0 |
