5JME
Crystal structure of acetylcholine binding protein (AChBP) from Aplysia Californica in complex with alpha-conotoxin PeIA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 145.115, 147.890, 146.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.821 - 2.336 |
| R-factor | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ez1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.821 |
| High resolution limit [Å] | 2.336 |
| Number of reflections | 66090 |
| <I/σ(I)> | 3.51 |
| Completeness [%] | 92.6 |
| Redundancy | 14.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.1 M Tris-HCl, 0.25 M magnesium chloride, 20% w/v PEG4000 |
