5JLW

AntpHD with 15bp DNA duplex R-monothioated at Cytidine-8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-12-09
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5418
Spacegroup name	P 2 2 21
Unit cell lengths	61.266, 75.888, 93.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.298 - 2.088
R-factor	0.2193
Rwork	0.218
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xid
RMSD bond length	0.009
RMSD bond angle	1.117
Data reduction software	SAINT
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.300	2.190
High resolution limit [Å]	2.088	2.090
Rmerge	0.079	0.503
Number of reflections	48110
<I/σ(I)>	11.99	1.84
Completeness [%]	97.5	92.1
Redundancy	5.72	4.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	20mM bis-Tris propane, 10mM NiCl2, 4-6% 2-methyl-2,4-pentanediol