5JLD

Crystal Structure of Arginyl-tRNA Synthetase from Plasmodium falciparum (PfRRS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-01
Detector	MARRESEARCH
Wavelength(s)	0.97903
Spacegroup name	P 1 21 1
Unit cell lengths	46.196, 62.079, 114.640
Unit cell angles	90.00, 96.05, 90.00

Refinement procedure

Resolution	41.987 - 2.200
R-factor	0.1874
Rwork	0.184
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q2y
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.059	0.034	0.557
Number of reflections	32304
<I/σ(I)>	7.7
Completeness [%]	98.4	97.3	97.8
Redundancy	4.6	4.4	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10.0%(W/V) PEG6000, 2.0%(W/V) PEG3350, 5%(V/V) MPD, 0.1M HEPES pH 7.5