5JK8
Phenylalanine hydroxylase from dictyostelium - BH2, norleucine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.2399 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.516, 85.668, 73.482 |
| Unit cell angles | 90.00, 110.08, 90.00 |
Refinement procedure
| Resolution | 34.742 - 2.394 |
| R-factor | 0.1689 |
| Rwork | 0.165 |
| R-free | 0.24190 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.390 |
| Rmerge | 0.090 |
| Number of reflections | 32512 |
| <I/σ(I)> | 15.4 |
| Completeness [%] | 98.6 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 26%(w/v) PEG 2000, 0.06 M PIPES |
