5JK4

Phosphate-Binding Protein from Stenotrophomonas maltophilia.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	37.729, 77.856, 56.287
Unit cell angles	90.00, 102.08, 90.00

Refinement procedure

Resolution	38.928 - 1.100
R-factor	0.1026
Rwork	0.102
R-free	0.12460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q9t
RMSD bond length	0.006
RMSD bond angle	0.961
Data reduction software	DIALS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.940	1.120
High resolution limit [Å]	1.100	1.100
Rmerge	0.076	0.631
Number of reflections	120995
<I/σ(I)>	9.7	2.3
Completeness [%]	94.1	85.9
Redundancy	4.4	3.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	15 % w/v PEG 8K, 100 mM TRIS pH 8.0, 10 mM sodium formate.