5JJQ
Crystal structure of IdnL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.750, 104.450, 120.000 |
| Unit cell angles | 81.78, 83.01, 81.98 |
Refinement procedure
| Resolution | 59.510 - 2.600 |
| R-factor | 0.23532 |
| Rwork | 0.234 |
| R-free | 0.26517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jjp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.704 |
| Data reduction software | iMOSFLM (1.0.7) |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.510 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.307 |
| Number of reflections | 90052 | |
| <I/σ(I)> | 6.3 | 2 |
| Completeness [%] | 94.1 | 94.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | PEG3350, sodium citrate, potassium chloride, ATP, magnesium chloride, (S)-3-aminobutyrate, Tris, glycerol |
