5JJK

Rho transcription termination factor bound to rA7 and 6 ADP-BeF3 molecules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.116
Spacegroup name	P 1 21 1
Unit cell lengths	68.760, 199.210, 112.200
Unit cell angles	90.00, 104.83, 90.00

Refinement procedure

Resolution	49.803 - 3.150
R-factor	0.235
Rwork	0.233
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jji
RMSD bond length	0.009
RMSD bond angle	0.560
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.803	3.260
High resolution limit [Å]	3.150	3.150
Rmerge	0.121	0.600
Number of reflections	49848
<I/σ(I)>	9.4	2
Completeness [%]	99.0	99.5
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291	200 mM KOAc, 40% MPD, 0.5% ethyl acetate