5JJ2

Crystal structure of the central domain of human AKAP18 gamma/delta in complex with malonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	56.250, 60.260, 65.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.360 - 1.250
R-factor	0.1618
Rwork	0.161
R-free	0.18030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vfy
RMSD bond length	0.005
RMSD bond angle	0.850
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (Version 1.10-2152)
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.360	1.280
High resolution limit [Å]	1.250	1.250
Rmerge	0.043
Number of reflections	119131
<I/σ(I)>	18.5	1.49
Completeness [%]	99.3	99.5
Redundancy	12.1	10.7
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	2.5 M Sodium malonate