5JIG
Crytsal structure of Wss1 from S. pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-09-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.300, 41.300, 68.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.000 |
| R-factor | 0.14265 |
| Rwork | 0.141 |
| R-free | 0.16724 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.913 |
| Data reduction software | XDS (20151015) |
| Data scaling software | XDS (20151015) |
| Phasing software | SHARP (2.8.5) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.100 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.059 | 0.399 |
| Number of reflections | 61720 | |
| <I/σ(I)> | 21 | 4.9 |
| Completeness [%] | 98.7 | 99.7 |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris/HCl, 20% PEG 1000 |
