5JHX

Crystal Structure of Fungal MagKatG2 at pH 3.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9729
Spacegroup name	P 21 21 21
Unit cell lengths	103.220, 109.860, 132.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.13911
Rwork	0.138
R-free	0.16100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut2
RMSD bond length	0.028
RMSD bond angle	2.494
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.440
High resolution limit [Å]	1.400	1.400
Rmerge	0.070	0.570
Number of reflections	293409
<I/σ(I)>	16.2	3.4
Completeness [%]	99.9	98.9
Redundancy	6.6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	15% PEG4000, 0.1 M sodium acetate, pH 4.6 (+ pH 3 sodium citrate soaking)