5JHQ
ARCs 1-3 of human Tankyrase-1 bound to a peptide derived from IRAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.510, 129.830, 123.950 |
Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.21514 |
Rwork | 0.214 |
R-free | 0.23947 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tww |
RMSD bond length | 0.008 |
RMSD bond angle | 1.301 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 48511 |
<I/σ(I)> | 11.8 |
Completeness [%] | 98.2 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 1.8 M (NH4)2SO4 100 mM Tris pH 8.5 |