5JHE
The Crystal Structure of the Saccharomyces cerevisiae Co-Chaperone Cpr7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 42 |
| Unit cell lengths | 75.332, 75.332, 100.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.920 - 1.800 |
| R-factor | 0.18597 |
| Rwork | 0.185 |
| R-free | 0.21082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ihg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.463 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.149 |
| Number of reflections | 51730 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 98.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 12% (w/v) PEG 8000, 0.1M Ca acetate, 0.1M Na cacodylate, pH 5.5 |
