5JGZ

Spin-Labeled T4 Lysozyme Construct T151V1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-11
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.460, 59.460, 95.302
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.730 - 1.534
R-factor	0.1693
Rwork	0.167
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c6t
RMSD bond length	0.006
RMSD bond angle	0.741
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.980
High resolution limit [Å]	1.534	1.534
Number of reflections	29805
<I/σ(I)>	11.46
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium/potassium phosphate, sodium chloride, hexane-1,6-diol, 2-propanol