5JGR

Spin-Labeled T4 Lysozyme Construct K43V1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	59.760, 59.760, 96.259
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.750 - 1.460
R-factor	0.1679
Rwork	0.166
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c6t
RMSD bond length	0.010
RMSD bond angle	0.769
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.750
High resolution limit [Å]	1.460	1.460
Number of reflections	34628
<I/σ(I)>	6.3
Completeness [%]	98.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	297	sodium/potassium phosphate, sodium chloride, hexane-1,6-diol, 2-propanol