5JGK
Crystal structure of GtmA in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.748, 109.301, 59.856 |
| Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
| Resolution | 45.117 - 1.330 |
| R-factor | 0.1401 |
| Rwork | 0.139 |
| R-free | 0.16640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.120 | 45.120 | 1.350 |
| High resolution limit [Å] | 1.330 | 7.280 | 1.330 |
| Rmerge | 0.064 | 0.040 | 0.896 |
| Rmeas | 0.070 | ||
| Rpim | 0.027 | ||
| Total number of observations | 852355 | ||
| Number of reflections | 130776 | ||
| <I/σ(I)> | 14.4 | ||
| Completeness [%] | 98.7 | 98.6 | 99.1 |
| Redundancy | 6.5 | 6.7 | 6.6 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.4 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M MES pH 6.0 |
