5JGC

Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A, L130A, L179A and I195A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 42 21 2
Unit cell lengths	57.516, 57.516, 174.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.319 - 2.080
R-factor	0.2115
Rwork	0.210
R-free	0.24570
RMSD bond length	0.005
RMSD bond angle	0.815
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.080	2.080
Rmerge	0.059	0.920
Number of reflections	18565
<I/σ(I)>	27.1	2.6
Completeness [%]	100.0	100
Redundancy	11.7	12
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	15% w/v Polyethylene glycol 4000, 0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride pH 8.5