5JFX
Crystal structure of TrkA in complex with PF-06273340
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 64 |
| Unit cell lengths | 125.013, 125.013, 46.216 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.860 - 1.630 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.23150 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.510 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.578 | |
| Number of reflections | 51246 | |
| <I/σ(I)> | 19.5 | 2.8 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 9.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | protein was concentrated to 10mg/ml. Crystals of complexes were grown using the hanging drop vapour diffusion method from 0.1M HEPES pH7.5, 0.1M MgCl2 and 10% EtOH at 4C |
