5JFG
Structure of humanised RadA-mutant humRadA22F in complex with peptide FHTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.515, 59.269, 88.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.070 - 1.770 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.19190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HumRadA4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.269 | 1.775 |
| High resolution limit [Å] | 1.769 | 1.769 |
| Rmerge | 0.082 | 0.841 |
| Number of reflections | 21412 | |
| <I/σ(I)> | 13.5 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.08M NaCacodylate pH=6.5, 0.16M CaAcetate, 18% PEG8000, 20% glycerol soaking: 5mM FHTA, 10% DMSO |
