5JEQ
Fragment of nitrate/nitrite sensor histidine kinase NarQ (R50K) in symmetric apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 39.593, 59.366, 239.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.640 - 1.900 |
| R-factor | 0.23424 |
| Rwork | 0.232 |
| R-free | 0.28498 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iji |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.640 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 1.230 |
| Number of reflections | 22889 | |
| <I/σ(I)> | 13.77 | 1.42 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.42 | 6.72 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | in meso lipidic cubic phase crystallization |
