5JEN
Crystal structure of the anti-sigma factor RsiV bound to lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2014-04-30 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 45.213, 130.326, 136.712 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.166 - 2.300 |
R-factor | 0.1837 |
Rwork | 0.181 |
R-free | 0.23660 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.159 |
Data reduction software | XDS (VERSION January 10, 2014) |
Data scaling software | XDS (VERSION January 10, 2014) |
Phasing software | PHENIX (1.10_2155) |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.530 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.109 | 0.899 |
Number of reflections | 36905 | |
<I/σ(I)> | 14 | 2.6 |
Completeness [%] | 98.9 | 99.9 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M sodium nitrate, 20% (w/v) PEG 3350. |