5JEI

Crystal structure of the GluA2 LBD in complex with FW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 21 21 2
Unit cell lengths	126.465, 44.425, 47.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.374 - 1.229
R-factor	0.122
Rwork	0.121
R-free	0.14730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftj
RMSD bond length	0.012
RMSD bond angle	1.492
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.374	1.300
High resolution limit [Å]	1.229	1.229
Rmerge		0.700
Rpim	0.069
Number of reflections	75826
<I/σ(I)>	14.52	2.26
Completeness [%]	96.7	92.8
Redundancy	4.07
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	277	50.0 % v/v PEG 200, 200 mM Sodium Chloride, 100 mM Sodium/Potassium Phosphate pH 6.2