5JEC

Apo-structure of humanised RadA-mutant humRadA33F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97631
Spacegroup name	P 31 2 1
Unit cell lengths	90.750, 90.750, 102.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.590 - 2.340
R-factor	0.201
Rwork	0.199
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j4l
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.594	2.348
High resolution limit [Å]	2.340	2.340
Rmerge	0.193	1.462
Number of reflections	21095
<I/σ(I)>	9.9	1.5
Completeness [%]	100.0	100
Redundancy	10.7	10.6
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	22% PEG3350, 0.1M BisTris pH=5.0, 0.2M Li2SO4