5JDB
Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-11-14 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.428, 114.931, 73.581 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 28.980 - 1.901 |
| R-factor | 0.2036 |
| Rwork | 0.202 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dwr |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 83986 |
| <I/σ(I)> | 17 |
| Completeness [%] | 97.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2.0 M ammonium sulfate |
