5JCE
Crystal structure of OsCEBiP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-04 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.448, 99.771, 87.647 |
Unit cell angles | 90.00, 103.73, 90.00 |
Refinement procedure
Resolution | 39.160 - 2.510 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jcd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.278 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 2.600 |
High resolution limit [Å] | 2.510 | 2.510 |
Number of reflections | 33694 | |
<I/σ(I)> | 17.2 | |
Completeness [%] | 97.6 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris, 2.0 M (NH4)2SO4 |