5JCA
NADP(H) bound NADH-dependent Ferredoxin:NADP Oxidoreductase (NfnI) from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.88 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 179.681, 179.681, 80.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.525 - 1.500 |
| R-factor | 0.1393 |
| Rwork | 0.139 |
| R-free | 0.15280 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.655 |
| Data reduction software | HKL-2000 (v712) |
| Data scaling software | SCALA (v712) |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2313: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.520 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | 0.557 |
| Number of reflections | 237751 | |
| <I/σ(I)> | 30.4 | 2.71 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 9.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1 M HEPES, 2.5 M Ammonium formate, 20% Glycerol |
