5JC9
Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 213.040, 436.850, 628.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.390 - 3.030 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.20000 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER-TNT (2.11.6) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.390 |
High resolution limit [Å] | 3.030 |
Number of reflections | 1084668 |
<I/σ(I)> | 10.8 |
Completeness [%] | 96.8 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 291 | PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES |