5JBS
Conformational changes during monomer-to-dimer transition of Brucella suis VirB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.503, 78.723, 70.840 |
| Unit cell angles | 90.00, 111.56, 90.00 |
Refinement procedure
| Resolution | 42.200 - 1.950 |
| R-factor | 0.19347 |
| Rwork | 0.191 |
| R-free | 0.24097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.900 |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.200 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 48835 |
| <I/σ(I)> | 2.4 |
| Completeness [%] | 97.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.1 M Tris/HCl pH 6.8, 18% PEG-MME 2000 |
