5JBS

Conformational changes during monomer-to-dimer transition of Brucella suis VirB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	67.503, 78.723, 70.840
Unit cell angles	90.00, 111.56, 90.00

Refinement procedure

Resolution	42.200 - 1.950
R-factor	0.19347
Rwork	0.191
R-free	0.24097
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.900
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.200
High resolution limit [Å]	1.950
Number of reflections	48835
<I/σ(I)>	2.4
Completeness [%]	97.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	0.1 M Tris/HCl pH 6.8, 18% PEG-MME 2000