5JBL
Structure of the bacteriophage T4 capsid assembly protease, gp21.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.745, 124.041, 88.959 |
Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
Resolution | 41.869 - 1.943 |
R-factor | 0.1731 |
Rwork | 0.172 |
R-free | 0.20470 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.852 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 1.980 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.097 | 0.540 |
Number of reflections | 61875 | |
<I/σ(I)> | 11.9 | 2 |
Completeness [%] | 93.0 | 76 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.3 M Ammonium sulfate, 0.1 M Hepes pH 7.5, and 0.1 M NaCl |