5JA2
EntF, a Terminal Nonribosomal Peptide Synthetase Module Bound to the non-Native MbtH-Like Protein PA2412
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 113.15 |
Detector technology | PIXEL |
Collection date | 2015-02-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 159.270, 57.690, 182.424 |
Unit cell angles | 90.00, 97.62, 90.00 |
Refinement procedure
Resolution | 58.974 - 3.000 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ja1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.547 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.980 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.103 | 0.621 |
Number of reflections | 31728 | |
<I/σ(I)> | 6.66 | 1.37 |
Completeness [%] | 94.9 | 90.53 |
Redundancy | 3.1 | 2.8 |
CC(1/2) | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287.15 | 100 mM Bis Tris propane, 50-150 mM MgCl2, and 15-28% Polyethylene glycol 4000 |