5JA1
EntF, a Terminal Nonribosomal Peptide Synthetase Module Bound to the MbtH-Like Protein YbdZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 113.15 |
Detector technology | CCD |
Collection date | 2015-10-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 161.185, 57.679, 183.945 |
Unit cell angles | 90.00, 97.93, 90.00 |
Refinement procedure
Resolution | 56.308 - 3.000 |
R-factor | 0.1879 |
Rwork | 0.185 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ZXJ |
RMSD bond length | 0.002 |
RMSD bond angle | 0.567 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.310 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.142 | 0.397 |
Number of reflections | 31781 | |
<I/σ(I)> | 7.05 | 3.14 |
Completeness [%] | 93.1 | 93.72 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287.15 | 100 mM Bis Tris propane, 50-150 mM MgCl2, and 15-28% Polyethylene glycol 4000 |