5J9S

ENL YEATS in complex with histone H3 acetylation at K27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-17
Detector	MARRESEARCH
Wavelength(s)	0.9790
Spacegroup name	P 31 2 1
Unit cell lengths	104.979, 104.979, 45.128
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.422 - 2.702
R-factor	0.1872
Rwork	0.185
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tmp
RMSD bond length	0.003
RMSD bond angle	0.611
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.234	0.099	0.809
Number of reflections	8062
<I/σ(I)>	4.1
Completeness [%]	99.7	97.3	100
Redundancy	6.4	6	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M K/Na Tartrate, 0.1M Sodium citrate tribasic dihydrate, pH 5.6, 2M (NH4)2SO4