5J9H

Crystal structure of Glycoprotein C from Puumala virus in the post-fusion conformation (pH 8.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	I 21 3
Unit cell lengths	138.521, 138.521, 138.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.500
R-factor	0.211
Rwork	0.208
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j81
RMSD bond length	0.008
RMSD bond angle	1.271
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.000		2.530
High resolution limit [Å]	2.500	20.000	2.500
Rmerge	0.072	0.042	0.783
Number of reflections	15391
<I/σ(I)>	14.43	31.82	2.15
Completeness [%]	99.6	86.8	99.6
Redundancy	5.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	40% PEG 400 0.2 M Lithium sulphate 0.1 M Tris buffer pH 8.0