5J97

Dimerization domain of cytoplasmic activation/proliferation-associated protein-2 (caprin-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	71.020, 71.020, 133.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.160 - 2.550
R-factor	0.214
Rwork	0.211
R-free	0.24400
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.990
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.158
High resolution limit [Å]	2.550	2.550
Number of reflections	11231
<I/σ(I)>	19.5
Completeness [%]	96.6
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	20% PEG8000, 0.1 M Tris (pH8.0), 10% glycerol