5J8Q
Crystal Structure of the Cysteine Desulfurase SufS of Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 93.191, 93.191, 130.543 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.600 - 1.702 |
| R-factor | 0.11771 |
| Rwork | 0.116 |
| R-free | 0.14789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w91 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.584 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.762 |
| High resolution limit [Å] | 1.702 | 1.702 |
| Rmerge | 0.071 | |
| Number of reflections | 72121 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 97.0 | |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 50 % (v/v) PEG 400 |
