5J8P

Lys27-linked diubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	44.828, 63.411, 52.844
Unit cell angles	90.00, 109.16, 90.00

Refinement procedure

Resolution	31.706 - 1.554
R-factor	0.2368
Rwork	0.234
R-free	0.29460
RMSD bond length	0.012
RMSD bond angle	1.466
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	31.706
High resolution limit [Å]	1.554
Number of reflections	13686
<I/σ(I)>	13.2
Completeness [%]	67.9
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M magnesium acetate tetrahydrate, 0.1M sodium cacodylate trihydrate, pH 6.5, 20% PEG 8000