5J7T

Molecular Understanding of USP7 Substrate Recognition and C-Terminal Activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9796
Spacegroup name	F 2 2 2
Unit cell lengths	104.330, 196.730, 226.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.376 - 3.200
R-factor	0.2519
Rwork	0.250
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nb8
RMSD bond length	0.002
RMSD bond angle	0.579
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.245	74.245	3.211
High resolution limit [Å]	3.200	14.815	3.200
Rmerge	0.128	0.076	0.670
Rmeas	0.144
Rpim	0.066
Total number of observations	91597
Number of reflections	19259
<I/σ(I)>	7.6
Completeness [%]	99.2	71.7	100
Redundancy	4.8	3.6	5
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	289	PEG 3350, 0.2M tri-potassium citrate