5J77
Mutant glyceraldehyde dehydrogenase (F34M+S405N) from Thermoplasma acidophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.071, 158.423, 130.050 |
Unit cell angles | 90.00, 91.57, 90.00 |
Refinement procedure
Resolution | 44.630 - 2.100 |
R-factor | 0.17843 |
Rwork | 0.176 |
R-free | 0.22631 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5izd |
RMSD bond length | 0.016 |
RMSD bond angle | 1.685 |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | XDS (November 3, 2014) |
Phasing software | MOLREP (11.2.08) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.630 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.092 | |
Number of reflections | 126605 | |
<I/σ(I)> | 12.13 | 2.32 |
Completeness [%] | 99.6 | 98.7 |
Redundancy | 3.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 0.1 M sodium acetate pH 4.6, 40%(v/v) PEG 200 |