5J6Y

Crystal structure of PA14 domain of MpAFP Antifreeze protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	93
Detector technology	CCD
Collection date	2015-09-15
Detector	RAYONIX MX300HE
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	45.220, 50.550, 79.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.710 - 1.030
R-factor	0.156
Rwork	0.155
R-free	0.16400
RMSD bond length	0.008
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.220	1.050
High resolution limit [Å]	1.030	1.030
Rmerge	0.042
Number of reflections	74600
<I/σ(I)>	23.9
Completeness [%]	83.2
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	20% PEG 3350, 0.2 M Calcium Chloride, Glucose, HEPES pH7