5J6G
Recognition of the MHC class Ib molecule H2-Q10 by the natural killer cell receptor Ly49C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9436 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 101.010, 57.800, 210.704 |
Unit cell angles | 90.00, 96.15, 90.00 |
Refinement procedure
Resolution | 46.160 - 3.300 |
R-factor | 0.2224 |
Rwork | 0.221 |
R-free | 0.25320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.830 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.160 |
High resolution limit [Å] | 3.300 |
Number of reflections | 18559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 12% Peg8000 10% Peg400 0.1M HEPES ph7.2 0.2M NaCl |