5J69
Structure of Astrotactin-2, a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.700, 86.600, 108.360 |
| Unit cell angles | 90.00, 111.74, 90.00 |
Refinement procedure
| Resolution | 60.770 - 3.630 |
| R-factor | 0.2807 |
| Rwork | 0.280 |
| R-free | 0.30170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j67 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.669 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.770 | |
| High resolution limit [Å] | 3.630 | 3.630 |
| Rmerge | 0.188 | 1.547 |
| Number of reflections | 9653 | |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 49.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Citric buffer pH 4.0 to 4.5 20% PEG 1000 |
