5J68
Structure of Astrotactin-2, a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 103.900, 103.900, 304.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.802 - 5.221 |
R-factor | 0.3483 |
Rwork | 0.348 |
R-free | 0.36450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j67 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.841 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.802 | |
High resolution limit [Å] | 5.220 | 5.220 |
Rmerge | 0.120 | 3.047 |
Number of reflections | 6915 | |
<I/σ(I)> | 17.3 | |
Completeness [%] | 100.0 | |
Redundancy | 23.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES, pH 5.0 to 6.0, 5% PEG 6000 |