5J59

Trypanosoma brucei methionyl-tRNA synthetase in complex with inhibitor (Chem 1893)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-27
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	87.310, 106.400, 207.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.960 - 2.400
R-factor	0.19245
Rwork	0.192
R-free	0.20745
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.231
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	103.960
High resolution limit [Å]	2.400
Number of reflections	75745
<I/σ(I)>	8.4
Completeness [%]	99.2
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.8-2.1 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M sodium cacodylate, pH 6.2-6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K